Notice (8): file_put_contents(): Write of 268 bytes failed with errno=28 No space left on device [CORE/src/Log/Engine/FileLog.php, line 140]

        if (!$mask) {
            file_put_contents($pathname, $message . "\n", FILE_APPEND);

file_put_contents - [internal], line ??
Cake\Log\Engine\FileLog::log() - CORE/src/Log/Engine/FileLog.php, line 140
Cake\Log\Log::write() - CORE/src/Log/Log.php, line 392
Cake\Log\Log::warning() - CORE/src/Log/Log.php, line 477
DebugKit\ToolbarService::isSuspiciouslyProduction() - ROOT/vendor/cakephp/debug_kit/src/ToolbarService.php, line 169
DebugKit\ToolbarService::isEnabled() - ROOT/vendor/cakephp/debug_kit/src/ToolbarService.php, line 105
DebugKit\Plugin::bootstrap() - ROOT/vendor/cakephp/debug_kit/src/Plugin.php, line 48
Cake\Http\BaseApplication::pluginBootstrap() - CORE/src/Http/BaseApplication.php, line 182
Cake\Http\Server::bootstrap() - CORE/src/Http/Server.php, line 111
Cake\Http\Server::run() - CORE/src/Http/Server.php, line 79
[main] - ROOT/webroot/index.php, line 40

Notice (8): SplFileObject::fwrite() [<a href='https://secure.php.net/splfileobject.fwrite'>splfileobject.fwrite</a>]: Write of 5131 bytes failed with errno=28 No space left on device [CORE/src/Cache/Engine/FileEngine.php, line 141]

Code Context

        $success = $this->_File->ftruncate(0) &&
            $this->_File->fwrite($contents) &&
            $this->_File->fflush();

SplFileObject::fwrite() - [internal], line ??
Cake\Cache\Engine\FileEngine::set() - CORE/src/Cache/Engine/FileEngine.php, line 141
Cake\Database\Schema\CachedCollection::describe() - CORE/src/Database/Schema/CachedCollection.php, line 85
Cake\ORM\Table::getSchema() - CORE/src/ORM/Table.php, line 513
Cake\ORM\Query::addDefaultTypes() - CORE/src/ORM/Query.php, line 290
Cake\ORM\Query::__construct() - CORE/src/ORM/Query.php, line 184
Cake\ORM\Table::query() - CORE/src/ORM/Table.php, line 1702
Cake\ORM\Table::find() - CORE/src/ORM/Table.php, line 1263
App\Controller\NewsController::action_() - APP/Controller/NewsController.php, line 6437
App\Controller\NewsController::get_data() - APP/Controller/NewsController.php, line 5701
App\Controller\NewsController::action_cache() - APP/Controller/NewsController.php, line 5625
App\Controller\NewsController::cache_action() - APP/Controller/NewsController.php, line 5281
App\Controller\NewsController::view() - APP/Controller/NewsController.php, line 3226
Cake\Controller\Controller::invokeAction() - CORE/src/Controller/Controller.php, line 539
Cake\Controller\ControllerFactory::handle() - CORE/src/Controller/ControllerFactory.php, line 140
Cake\Controller\ControllerFactory::invoke() - CORE/src/Controller/ControllerFactory.php, line 115
Cake\Http\BaseApplication::handle() - CORE/src/Http/BaseApplication.php, line 317

Notice (8): unserialize() [<a href='https://secure.php.net/function.unserialize'>function.unserialize</a>]: Error at offset 16363 of 16373 bytes [APP/Controller/NewsController.php, line 5571]

Code Context

               {
                      $a=unserialize($line2);
               }

unserialize - [internal], line ??
App\Controller\NewsController::action_cache() - APP/Controller/NewsController.php, line 5571
App\Controller\NewsController::cache_action() - APP/Controller/NewsController.php, line 5281
App\Controller\NewsController::action_() - APP/Controller/NewsController.php, line 6481
App\Controller\NewsController::get_data() - APP/Controller/NewsController.php, line 5701
App\Controller\NewsController::action_cache() - APP/Controller/NewsController.php, line 5625
App\Controller\NewsController::cache_action() - APP/Controller/NewsController.php, line 5281
App\Controller\NewsController::view() - APP/Controller/NewsController.php, line 3436
Cake\Controller\Controller::invokeAction() - CORE/src/Controller/Controller.php, line 539
Cake\Controller\ControllerFactory::handle() - CORE/src/Controller/ControllerFactory.php, line 140
Cake\Controller\ControllerFactory::invoke() - CORE/src/Controller/ControllerFactory.php, line 115
Cake\Http\BaseApplication::handle() - CORE/src/Http/BaseApplication.php, line 317
Cake\Http\Runner::handle() - CORE/src/Http/Runner.php, line 77
Cake\Http\Middleware\CsrfProtectionMiddleware::process() - CORE/src/Http/Middleware/CsrfProtectionMiddleware.php, line 164
Cake\Http\Runner::handle() - CORE/src/Http/Runner.php, line 73
Cake\I18n\Middleware\LocaleSelectorMiddleware::process() - CORE/src/I18n/Middleware/LocaleSelectorMiddleware.php, line 61
Cake\Http\Runner::handle() - CORE/src/Http/Runner.php, line 73

Warning (2): Undefined array key "nsort" [APP/Controller/NewsController.php, line 3613]

Code Context

                
                $nav_url.="<a href='".$website_info[0]["nsort"]["nsort_url"]."'>".$website_info[0]["nsort"]["nsort_name"]."</a>";

App\Controller\NewsController::view() - APP/Controller/NewsController.php, line 3613
Cake\Controller\Controller::invokeAction() - CORE/src/Controller/Controller.php, line 539
Cake\Controller\ControllerFactory::handle() - CORE/src/Controller/ControllerFactory.php, line 140
Cake\Controller\ControllerFactory::invoke() - CORE/src/Controller/ControllerFactory.php, line 115
Cake\Http\BaseApplication::handle() - CORE/src/Http/BaseApplication.php, line 317
Cake\Http\Runner::handle() - CORE/src/Http/Runner.php, line 77
Cake\Http\Middleware\CsrfProtectionMiddleware::process() - CORE/src/Http/Middleware/CsrfProtectionMiddleware.php, line 164
Cake\Http\Runner::handle() - CORE/src/Http/Runner.php, line 73
Cake\I18n\Middleware\LocaleSelectorMiddleware::process() - CORE/src/I18n/Middleware/LocaleSelectorMiddleware.php, line 61
Cake\Http\Runner::handle() - CORE/src/Http/Runner.php, line 73
Cake\Http\Middleware\BodyParserMiddleware::process() - CORE/src/Http/Middleware/BodyParserMiddleware.php, line 157
Cake\Http\Runner::handle() - CORE/src/Http/Runner.php, line 73
Cake\Routing\Middleware\RoutingMiddleware::process() - CORE/src/Routing/Middleware/RoutingMiddleware.php, line 161
Cake\Http\Runner::handle() - CORE/src/Http/Runner.php, line 73
Cake\Routing\Middleware\AssetMiddleware::process() - CORE/src/Routing/Middleware/AssetMiddleware.php, line 77
Cake\Http\Runner::handle() - CORE/src/Http/Runner.php, line 73
Cake\Error\Middleware\ErrorHandlerMiddleware::process() - CORE/src/Error/Middleware/ErrorHandlerMiddleware.php, line 126

Warning (2): Trying to access array offset on value of type null [APP/Controller/NewsController.php, line 3613]

Code Context

                
                $nav_url.="<a href='".$website_info[0]["nsort"]["nsort_url"]."'>".$website_info[0]["nsort"]["nsort_name"]."</a>";

App\Controller\NewsController::view() - APP/Controller/NewsController.php, line 3613
Cake\Controller\Controller::invokeAction() - CORE/src/Controller/Controller.php, line 539
Cake\Controller\ControllerFactory::handle() - CORE/src/Controller/ControllerFactory.php, line 140
Cake\Controller\ControllerFactory::invoke() - CORE/src/Controller/ControllerFactory.php, line 115
Cake\Http\BaseApplication::handle() - CORE/src/Http/BaseApplication.php, line 317
Cake\Http\Runner::handle() - CORE/src/Http/Runner.php, line 77
Cake\Http\Middleware\CsrfProtectionMiddleware::process() - CORE/src/Http/Middleware/CsrfProtectionMiddleware.php, line 164
Cake\Http\Runner::handle() - CORE/src/Http/Runner.php, line 73
Cake\I18n\Middleware\LocaleSelectorMiddleware::process() - CORE/src/I18n/Middleware/LocaleSelectorMiddleware.php, line 61
Cake\Http\Runner::handle() - CORE/src/Http/Runner.php, line 73
Cake\Http\Middleware\BodyParserMiddleware::process() - CORE/src/Http/Middleware/BodyParserMiddleware.php, line 157
Cake\Http\Runner::handle() - CORE/src/Http/Runner.php, line 73
Cake\Routing\Middleware\RoutingMiddleware::process() - CORE/src/Routing/Middleware/RoutingMiddleware.php, line 161
Cake\Http\Runner::handle() - CORE/src/Http/Runner.php, line 73
Cake\Routing\Middleware\AssetMiddleware::process() - CORE/src/Routing/Middleware/AssetMiddleware.php, line 77
Cake\Http\Runner::handle() - CORE/src/Http/Runner.php, line 73
Cake\Error\Middleware\ErrorHandlerMiddleware::process() - CORE/src/Error/Middleware/ErrorHandlerMiddleware.php, line 126

Warning (2): Undefined array key "nsort" [APP/Controller/NewsController.php, line 3613]

Code Context

                
                $nav_url.="<a href='".$website_info[0]["nsort"]["nsort_url"]."'>".$website_info[0]["nsort"]["nsort_name"]."</a>";

App\Controller\NewsController::view() - APP/Controller/NewsController.php, line 3613
Cake\Controller\Controller::invokeAction() - CORE/src/Controller/Controller.php, line 539
Cake\Controller\ControllerFactory::handle() - CORE/src/Controller/ControllerFactory.php, line 140
Cake\Controller\ControllerFactory::invoke() - CORE/src/Controller/ControllerFactory.php, line 115
Cake\Http\BaseApplication::handle() - CORE/src/Http/BaseApplication.php, line 317
Cake\Http\Runner::handle() - CORE/src/Http/Runner.php, line 77
Cake\Http\Middleware\CsrfProtectionMiddleware::process() - CORE/src/Http/Middleware/CsrfProtectionMiddleware.php, line 164
Cake\Http\Runner::handle() - CORE/src/Http/Runner.php, line 73
Cake\I18n\Middleware\LocaleSelectorMiddleware::process() - CORE/src/I18n/Middleware/LocaleSelectorMiddleware.php, line 61
Cake\Http\Runner::handle() - CORE/src/Http/Runner.php, line 73
Cake\Http\Middleware\BodyParserMiddleware::process() - CORE/src/Http/Middleware/BodyParserMiddleware.php, line 157
Cake\Http\Runner::handle() - CORE/src/Http/Runner.php, line 73
Cake\Routing\Middleware\RoutingMiddleware::process() - CORE/src/Routing/Middleware/RoutingMiddleware.php, line 161
Cake\Http\Runner::handle() - CORE/src/Http/Runner.php, line 73
Cake\Routing\Middleware\AssetMiddleware::process() - CORE/src/Routing/Middleware/AssetMiddleware.php, line 77
Cake\Http\Runner::handle() - CORE/src/Http/Runner.php, line 73
Cake\Error\Middleware\ErrorHandlerMiddleware::process() - CORE/src/Error/Middleware/ErrorHandlerMiddleware.php, line 126

Warning (2): Trying to access array offset on value of type null [APP/Controller/NewsController.php, line 3613]

Code Context

                
                $nav_url.="<a href='".$website_info[0]["nsort"]["nsort_url"]."'>".$website_info[0]["nsort"]["nsort_name"]."</a>";

App\Controller\NewsController::view() - APP/Controller/NewsController.php, line 3613
Cake\Controller\Controller::invokeAction() - CORE/src/Controller/Controller.php, line 539
Cake\Controller\ControllerFactory::handle() - CORE/src/Controller/ControllerFactory.php, line 140
Cake\Controller\ControllerFactory::invoke() - CORE/src/Controller/ControllerFactory.php, line 115
Cake\Http\BaseApplication::handle() - CORE/src/Http/BaseApplication.php, line 317
Cake\Http\Runner::handle() - CORE/src/Http/Runner.php, line 77
Cake\Http\Middleware\CsrfProtectionMiddleware::process() - CORE/src/Http/Middleware/CsrfProtectionMiddleware.php, line 164
Cake\Http\Runner::handle() - CORE/src/Http/Runner.php, line 73
Cake\I18n\Middleware\LocaleSelectorMiddleware::process() - CORE/src/I18n/Middleware/LocaleSelectorMiddleware.php, line 61
Cake\Http\Runner::handle() - CORE/src/Http/Runner.php, line 73
Cake\Http\Middleware\BodyParserMiddleware::process() - CORE/src/Http/Middleware/BodyParserMiddleware.php, line 157
Cake\Http\Runner::handle() - CORE/src/Http/Runner.php, line 73
Cake\Routing\Middleware\RoutingMiddleware::process() - CORE/src/Routing/Middleware/RoutingMiddleware.php, line 161
Cake\Http\Runner::handle() - CORE/src/Http/Runner.php, line 73
Cake\Routing\Middleware\AssetMiddleware::process() - CORE/src/Routing/Middleware/AssetMiddleware.php, line 77
Cake\Http\Runner::handle() - CORE/src/Http/Runner.php, line 73
Cake\Error\Middleware\ErrorHandlerMiddleware::process() - CORE/src/Error/Middleware/ErrorHandlerMiddleware.php, line 126

Notice (8): SplFileObject::fwrite() [<a href='https://secure.php.net/splfileobject.fwrite'>splfileobject.fwrite</a>]: Write of 134 bytes failed with errno=28 No space left on device [CORE/src/Cache/Engine/FileEngine.php, line 141]

Code Context

        $success = $this->_File->ftruncate(0) &&
            $this->_File->fwrite($contents) &&
            $this->_File->fflush();

SplFileObject::fwrite() - [internal], line ??
Cake\Cache\Engine\FileEngine::set() - CORE/src/Cache/Engine/FileEngine.php, line 141
Cake\Cache\Cache::write() - CORE/src/Cache/Cache.php, line 266
App\Controller\NewsController::action_() - APP/Controller/NewsController.php, line 6766
App\Controller\NewsController::get_data() - APP/Controller/NewsController.php, line 5701
App\Controller\NewsController::action_cache() - APP/Controller/NewsController.php, line 5625
App\Controller\NewsController::cache_action() - APP/Controller/NewsController.php, line 5281
App\Controller\NewsController::view() - APP/Controller/NewsController.php, line 3634
Cake\Controller\Controller::invokeAction() - CORE/src/Controller/Controller.php, line 539
Cake\Controller\ControllerFactory::handle() - CORE/src/Controller/ControllerFactory.php, line 140
Cake\Controller\ControllerFactory::invoke() - CORE/src/Controller/ControllerFactory.php, line 115
Cake\Http\BaseApplication::handle() - CORE/src/Http/BaseApplication.php, line 317
Cake\Http\Runner::handle() - CORE/src/Http/Runner.php, line 77
Cake\Http\Middleware\CsrfProtectionMiddleware::process() - CORE/src/Http/Middleware/CsrfProtectionMiddleware.php, line 164
Cake\Http\Runner::handle() - CORE/src/Http/Runner.php, line 73
Cake\I18n\Middleware\LocaleSelectorMiddleware::process() - CORE/src/I18n/Middleware/LocaleSelectorMiddleware.php, line 61
Cake\Http\Runner::handle() - CORE/src/Http/Runner.php, line 73

Warning (512): long cache was unable to write '9f75b5ef7afc1a4fd574973db96f53f4' to Cake\Cache\Engine\FileEngine cache [CORE/src/Cache/Cache.php, line 275]

Code Context

                ),
                E_USER_WARNING
            );

Cake\Cache\Cache::write() - CORE/src/Cache/Cache.php, line 275
App\Controller\NewsController::action_() - APP/Controller/NewsController.php, line 6766
App\Controller\NewsController::get_data() - APP/Controller/NewsController.php, line 5701
App\Controller\NewsController::action_cache() - APP/Controller/NewsController.php, line 5625
App\Controller\NewsController::cache_action() - APP/Controller/NewsController.php, line 5281
App\Controller\NewsController::view() - APP/Controller/NewsController.php, line 3634
Cake\Controller\Controller::invokeAction() - CORE/src/Controller/Controller.php, line 539
Cake\Controller\ControllerFactory::handle() - CORE/src/Controller/ControllerFactory.php, line 140
Cake\Controller\ControllerFactory::invoke() - CORE/src/Controller/ControllerFactory.php, line 115
Cake\Http\BaseApplication::handle() - CORE/src/Http/BaseApplication.php, line 317
Cake\Http\Runner::handle() - CORE/src/Http/Runner.php, line 77
Cake\Http\Middleware\CsrfProtectionMiddleware::process() - CORE/src/Http/Middleware/CsrfProtectionMiddleware.php, line 164
Cake\Http\Runner::handle() - CORE/src/Http/Runner.php, line 73
Cake\I18n\Middleware\LocaleSelectorMiddleware::process() - CORE/src/I18n/Middleware/LocaleSelectorMiddleware.php, line 61
Cake\Http\Runner::handle() - CORE/src/Http/Runner.php, line 73
Cake\Http\Middleware\BodyParserMiddleware::process() - CORE/src/Http/Middleware/BodyParserMiddleware.php, line 157

Notice (8): SplFileObject::fwrite() [<a href='https://secure.php.net/splfileobject.fwrite'>splfileobject.fwrite</a>]: Write of 109 bytes failed with errno=28 No space left on device [CORE/src/Cache/Engine/FileEngine.php, line 141]

Code Context

        $success = $this->_File->ftruncate(0) &&
            $this->_File->fwrite($contents) &&
            $this->_File->fflush();

SplFileObject::fwrite() - [internal], line ??
Cake\Cache\Engine\FileEngine::set() - CORE/src/Cache/Engine/FileEngine.php, line 141
Cake\Cache\Cache::write() - CORE/src/Cache/Cache.php, line 266
App\Controller\NewsController::action_() - APP/Controller/NewsController.php, line 6766
App\Controller\NewsController::get_data() - APP/Controller/NewsController.php, line 5701
App\Controller\NewsController::action_cache() - APP/Controller/NewsController.php, line 5625
App\Controller\NewsController::cache_action() - APP/Controller/NewsController.php, line 5281
App\Controller\NewsController::view() - APP/Controller/NewsController.php, line 3663
Cake\Controller\Controller::invokeAction() - CORE/src/Controller/Controller.php, line 539
Cake\Controller\ControllerFactory::handle() - CORE/src/Controller/ControllerFactory.php, line 140
Cake\Controller\ControllerFactory::invoke() - CORE/src/Controller/ControllerFactory.php, line 115
Cake\Http\BaseApplication::handle() - CORE/src/Http/BaseApplication.php, line 317
Cake\Http\Runner::handle() - CORE/src/Http/Runner.php, line 77
Cake\Http\Middleware\CsrfProtectionMiddleware::process() - CORE/src/Http/Middleware/CsrfProtectionMiddleware.php, line 164
Cake\Http\Runner::handle() - CORE/src/Http/Runner.php, line 73
Cake\I18n\Middleware\LocaleSelectorMiddleware::process() - CORE/src/I18n/Middleware/LocaleSelectorMiddleware.php, line 61
Cake\Http\Runner::handle() - CORE/src/Http/Runner.php, line 73

Warning (512): long cache was unable to write '7bc2f47924150986a1fcfc13b8324f62' to Cake\Cache\Engine\FileEngine cache [CORE/src/Cache/Cache.php, line 275]

Code Context

                ),
                E_USER_WARNING
            );

Cake\Cache\Cache::write() - CORE/src/Cache/Cache.php, line 275
App\Controller\NewsController::action_() - APP/Controller/NewsController.php, line 6766
App\Controller\NewsController::get_data() - APP/Controller/NewsController.php, line 5701
App\Controller\NewsController::action_cache() - APP/Controller/NewsController.php, line 5625
App\Controller\NewsController::cache_action() - APP/Controller/NewsController.php, line 5281
App\Controller\NewsController::view() - APP/Controller/NewsController.php, line 3663
Cake\Controller\Controller::invokeAction() - CORE/src/Controller/Controller.php, line 539
Cake\Controller\ControllerFactory::handle() - CORE/src/Controller/ControllerFactory.php, line 140
Cake\Controller\ControllerFactory::invoke() - CORE/src/Controller/ControllerFactory.php, line 115
Cake\Http\BaseApplication::handle() - CORE/src/Http/BaseApplication.php, line 317
Cake\Http\Runner::handle() - CORE/src/Http/Runner.php, line 77
Cake\Http\Middleware\CsrfProtectionMiddleware::process() - CORE/src/Http/Middleware/CsrfProtectionMiddleware.php, line 164
Cake\Http\Runner::handle() - CORE/src/Http/Runner.php, line 73
Cake\I18n\Middleware\LocaleSelectorMiddleware::process() - CORE/src/I18n/Middleware/LocaleSelectorMiddleware.php, line 61
Cake\Http\Runner::handle() - CORE/src/Http/Runner.php, line 73
Cake\Http\Middleware\BodyParserMiddleware::process() - CORE/src/Http/Middleware/BodyParserMiddleware.php, line 157

Notice (8): unserialize() [<a href='https://secure.php.net/function.unserialize'>function.unserialize</a>]: Error at offset 16147 of 16373 bytes [APP/Controller/NewsController.php, line 5571]

Code Context

               {
                      $a=unserialize($line2);
               }

unserialize - [internal], line ??
App\Controller\NewsController::action_cache() - APP/Controller/NewsController.php, line 5571
App\Controller\NewsController::cache_action() - APP/Controller/NewsController.php, line 5281
App\Controller\NewsController::view() - APP/Controller/NewsController.php, line 3698
Cake\Controller\Controller::invokeAction() - CORE/src/Controller/Controller.php, line 539
Cake\Controller\ControllerFactory::handle() - CORE/src/Controller/ControllerFactory.php, line 140
Cake\Controller\ControllerFactory::invoke() - CORE/src/Controller/ControllerFactory.php, line 115
Cake\Http\BaseApplication::handle() - CORE/src/Http/BaseApplication.php, line 317
Cake\Http\Runner::handle() - CORE/src/Http/Runner.php, line 77
Cake\Http\Middleware\CsrfProtectionMiddleware::process() - CORE/src/Http/Middleware/CsrfProtectionMiddleware.php, line 164
Cake\Http\Runner::handle() - CORE/src/Http/Runner.php, line 73
Cake\I18n\Middleware\LocaleSelectorMiddleware::process() - CORE/src/I18n/Middleware/LocaleSelectorMiddleware.php, line 61
Cake\Http\Runner::handle() - CORE/src/Http/Runner.php, line 73
Cake\Http\Middleware\BodyParserMiddleware::process() - CORE/src/Http/Middleware/BodyParserMiddleware.php, line 157
Cake\Http\Runner::handle() - CORE/src/Http/Runner.php, line 73
Cake\Routing\Middleware\RoutingMiddleware::process() - CORE/src/Routing/Middleware/RoutingMiddleware.php, line 161
Cake\Http\Runner::handle() - CORE/src/Http/Runner.php, line 73

高分辨活体成像技术成功应用于活体胚胎研究

Warning (2): Undefined array key "nsort" [ROOT/plugins/Kuhuang/templates/Websites/view.php, line 430]

Code Context

          <div class="info"> 
            <span><a href="<?php echo $website_info[0]["nsort"]["nsort_url"];?>"><i class="fa fa-columns"></i><?php echo $website_info[0]["nsort"]["nsort_name"];?></a></span> 
            <span><i class="fa fa-clock-o"></i><?php echo $website_info[0]["submission_date"];?></span>

include - ROOT/plugins/Kuhuang/templates/Websites/view.php, line 430
Cake\View\View::_evaluate() - CORE/src/View/View.php, line 1184
Cake\View\View::_render() - CORE/src/View/View.php, line 1138
Cake\View\View::render() - CORE/src/View/View.php, line 769
Cake\Controller\Controller::render() - CORE/src/Controller/Controller.php, line 762
App\Controller\NewsController::view() - APP/Controller/NewsController.php, line 3938
Cake\Controller\Controller::invokeAction() - CORE/src/Controller/Controller.php, line 539
Cake\Controller\ControllerFactory::handle() - CORE/src/Controller/ControllerFactory.php, line 140
Cake\Controller\ControllerFactory::invoke() - CORE/src/Controller/ControllerFactory.php, line 115
Cake\Http\BaseApplication::handle() - CORE/src/Http/BaseApplication.php, line 317
Cake\Http\Runner::handle() - CORE/src/Http/Runner.php, line 77
Cake\Http\Middleware\CsrfProtectionMiddleware::process() - CORE/src/Http/Middleware/CsrfProtectionMiddleware.php, line 164
Cake\Http\Runner::handle() - CORE/src/Http/Runner.php, line 73
Cake\I18n\Middleware\LocaleSelectorMiddleware::process() - CORE/src/I18n/Middleware/LocaleSelectorMiddleware.php, line 61
Cake\Http\Runner::handle() - CORE/src/Http/Runner.php, line 73
Cake\Http\Middleware\BodyParserMiddleware::process() - CORE/src/Http/Middleware/BodyParserMiddleware.php, line 157
Cake\Http\Runner::handle() - CORE/src/Http/Runner.php, line 73

Notice: file_put_contents() [function.file-put-contents]: Write of 2423 bytes failed with errno=28 No space left on device in /www/wwwroot/www.adminso.com/vendor/cakephp/cakephp/src/Log/Engine/FileLog.php on line 140

Warning (2): Trying to access array offset on value of type null [ROOT/plugins/Kuhuang/templates/Websites/view.php, line 430]Code Context          <div class="info"> 
            <span><a href="<?php echo $website_info[0]["nsort"]["nsort_url"];?>"><i class="fa fa-columns"></i><?php echo $website_info[0]["nsort"]["nsort_name"];?></a></span> 
            <span><i class="fa fa-clock-o"></i><?php echo $website_info[0]["submission_date"];?></span> 
include - ROOT/plugins/Kuhuang/templates/Websites/view.php, line 430
Cake\View\View::_evaluate() - CORE/src/View/View.php, line 1184
Cake\View\View::_render() - CORE/src/View/View.php, line 1138
Cake\View\View::render() - CORE/src/View/View.php, line 769
Cake\Controller\Controller::render() - CORE/src/Controller/Controller.php, line 762
App\Controller\NewsController::view() - APP/Controller/NewsController.php, line 3938
Cake\Controller\Controller::invokeAction() - CORE/src/Controller/Controller.php, line 539
Cake\Controller\ControllerFactory::handle() - CORE/src/Controller/ControllerFactory.php, line 140
Cake\Controller\ControllerFactory::invoke() - CORE/src/Controller/ControllerFactory.php, line 115
Cake\Http\BaseApplication::handle() - CORE/src/Http/BaseApplication.php, line 317
Cake\Http\Runner::handle() - CORE/src/Http/Runner.php, line 77
Cake\Http\Middleware\CsrfProtectionMiddleware::process() - CORE/src/Http/Middleware/CsrfProtectionMiddleware.php, line 164
Cake\Http\Runner::handle() - CORE/src/Http/Runner.php, line 73
Cake\I18n\Middleware\LocaleSelectorMiddleware::process() - CORE/src/I18n/Middleware/LocaleSelectorMiddleware.php, line 61
Cake\Http\Runner::handle() - CORE/src/Http/Runner.php, line 73
Cake\Http\Middleware\BodyParserMiddleware::process() - CORE/src/Http/Middleware/BodyParserMiddleware.php, line 157
Cake\Http\Runner::handle() - CORE/src/Http/Runner.php, line 73

Notice: file_put_contents() [function.file-put-contents]: Write of 2447 bytes failed with errno=28 No space left on device in /www/wwwroot/www.adminso.com/vendor/cakephp/cakephp/src/Log/Engine/FileLog.php on line 140
">

Warning (2): Undefined array key "nsort" [ROOT/plugins/Kuhuang/templates/Websites/view.php, line 430]Code Context          <div class="info"> 
            <span><a href="<?php echo $website_info[0]["nsort"]["nsort_url"];?>"><i class="fa fa-columns"></i><?php echo $website_info[0]["nsort"]["nsort_name"];?></a></span> 
            <span><i class="fa fa-clock-o"></i><?php echo $website_info[0]["submission_date"];?></span> 
include - ROOT/plugins/Kuhuang/templates/Websites/view.php, line 430
Cake\View\View::_evaluate() - CORE/src/View/View.php, line 1184
Cake\View\View::_render() - CORE/src/View/View.php, line 1138
Cake\View\View::render() - CORE/src/View/View.php, line 769
Cake\Controller\Controller::render() - CORE/src/Controller/Controller.php, line 762
App\Controller\NewsController::view() - APP/Controller/NewsController.php, line 3938
Cake\Controller\Controller::invokeAction() - CORE/src/Controller/Controller.php, line 539
Cake\Controller\ControllerFactory::handle() - CORE/src/Controller/ControllerFactory.php, line 140
Cake\Controller\ControllerFactory::invoke() - CORE/src/Controller/ControllerFactory.php, line 115
Cake\Http\BaseApplication::handle() - CORE/src/Http/BaseApplication.php, line 317
Cake\Http\Runner::handle() - CORE/src/Http/Runner.php, line 77
Cake\Http\Middleware\CsrfProtectionMiddleware::process() - CORE/src/Http/Middleware/CsrfProtectionMiddleware.php, line 164
Cake\Http\Runner::handle() - CORE/src/Http/Runner.php, line 73
Cake\I18n\Middleware\LocaleSelectorMiddleware::process() - CORE/src/I18n/Middleware/LocaleSelectorMiddleware.php, line 61
Cake\Http\Runner::handle() - CORE/src/Http/Runner.php, line 73
Cake\Http\Middleware\BodyParserMiddleware::process() - CORE/src/Http/Middleware/BodyParserMiddleware.php, line 157
Cake\Http\Runner::handle() - CORE/src/Http/Runner.php, line 73

Warning (2): Trying to access array offset on value of type null [ROOT/plugins/Kuhuang/templates/Websites/view.php, line 430]Code Context          <div class="info"> 
            <span><a href="<?php echo $website_info[0]["nsort"]["nsort_url"];?>"><i class="fa fa-columns"></i><?php echo $website_info[0]["nsort"]["nsort_name"];?></a></span> 
            <span><i class="fa fa-clock-o"></i><?php echo $website_info[0]["submission_date"];?></span> 
include - ROOT/plugins/Kuhuang/templates/Websites/view.php, line 430
Cake\View\View::_evaluate() - CORE/src/View/View.php, line 1184
Cake\View\View::_render() - CORE/src/View/View.php, line 1138
Cake\View\View::render() - CORE/src/View/View.php, line 769
Cake\Controller\Controller::render() - CORE/src/Controller/Controller.php, line 762
App\Controller\NewsController::view() - APP/Controller/NewsController.php, line 3938
Cake\Controller\Controller::invokeAction() - CORE/src/Controller/Controller.php, line 539
Cake\Controller\ControllerFactory::handle() - CORE/src/Controller/ControllerFactory.php, line 140
Cake\Controller\ControllerFactory::invoke() - CORE/src/Controller/ControllerFactory.php, line 115
Cake\Http\BaseApplication::handle() - CORE/src/Http/BaseApplication.php, line 317
Cake\Http\Runner::handle() - CORE/src/Http/Runner.php, line 77
Cake\Http\Middleware\CsrfProtectionMiddleware::process() - CORE/src/Http/Middleware/CsrfProtectionMiddleware.php, line 164
Cake\Http\Runner::handle() - CORE/src/Http/Runner.php, line 73
Cake\I18n\Middleware\LocaleSelectorMiddleware::process() - CORE/src/I18n/Middleware/LocaleSelectorMiddleware.php, line 61
Cake\Http\Runner::handle() - CORE/src/Http/Runner.php, line 73
Cake\Http\Middleware\BodyParserMiddleware::process() - CORE/src/Http/Middleware/BodyParserMiddleware.php, line 157
Cake\Http\Runner::handle() - CORE/src/Http/Runner.php, line 73

新知科技日报讯（记者史俊斌通讯员买秋霞）近日出版的《科学》杂志刊登了西安交通大学第二附属医院小儿外科黄强副研究员与杜克大学、麻省理工学院的合作研究成果，此项研究实现了以高分辨成像直视活体哺乳类动物胚胎发育的动态过程，为准确研究胚胎发育过程中的各种科学问题打开了一扇“窗”。哺乳动物胚胎发育是一个动态复杂的过程，既往研究方法包括组织染色、超声、OCT（光学相干断层成像）、MRI（磁共振成像）等，但是均不能在细胞水平上观察胚胎发育的动态过程

新知

科技日报讯（记者史俊斌通讯员买秋霞）近日出版的《科学》杂志刊登了西安交通大学第二附属医院小儿外科黄强副研究员与杜克大学、麻省理工学院的合作研究成果，此项研究实现了以高分辨成像直视活体哺乳类动物胚胎发育的动态过程，为准确研究胚胎发育过程中的各种科学问题打开了一扇“窗”。

哺乳动物胚胎发育是一个动态复杂的过程，既往研究方法包括组织染色、超声、OCT（光学相干断层成像）、MRI（磁共振成像）等，但是均不能在细胞水平上观察胚胎发育的动态过程。

近年来，黄强研究团队研究并开发了一种小鼠胚胎发育活体成像技术，通过不同发育阶段的“腹窗”，高分辨成像观察小鼠胚胎第9.5天至出生的连续发育过程。利用这项新技术，研究团队在转基因小鼠体内观察到了神经递质传递、大脑形成早期神经嵴细胞分化的血管周细胞、视网膜发育过程中的细胞自噬、腺病毒递送以及胎盘荧光化学药物转运等。通过与子宫内电转技术结合在大脑中标记特定细胞，观察了细胞分裂及迁移。随后与麻省理工学院合作，在同一人鼠嵌合体中追踪了人神经嵴细胞和鼠神经嵴细胞的嵌合差异。

黄强介绍，该方法实现了像研究透明的斑马鱼一样观察哺乳动物的胚胎发育过程，为活体直接观察，较体外培养保真度更高，实现了直视下观察哺乳动物胚胎发育、以高分辨成像活体观察胚胎发育的动态过程，有助于研究早期器官形成过程中不同干细胞的命运决定，并进行细胞谱系分析。结合其他生物医学技术，可以修饰、编辑胚胎发育中的特定细胞并研究该细胞的生物学特性，为基因治疗提供了一个研究模型。此外，通过构建人鼠嵌合体，还可以对人类干细胞及前体细胞进行研究。

标签: 活体高分辨成像技术成功应用于胚胎研究

声明：本文内容来源自网络，文字、图片等素材版权属于原作者，平台转载素材出于传递更多信息，文章内容仅供参考与学习，切勿作为商业目的使用。如果侵害了您的合法权益，请您及时与我们联系，我们会在第一时间进行处理！我们尊重版权，也致力于保护版权，站搜网感谢您的分享！

上一篇: 打车需求几乎停止，Lyft推出食品和杂货配送服务

上一篇: 西北华北等地有大风沙尘天气

娱乐休闲

工商与经济

电脑与网络

公司与企业

教育与培训

文学

艺术

体育与健身

新闻与媒体

卫生与健康

科学/文化

生活与服务

传媒人物

地方新闻媒体

社会性别研究

图书/书店

展览/活动

农村研究

新闻学

电脑门诊

博客/空间

运动常识

医药法规

家用电器

高分辨活体成像技术成功应用于活体胚胎研究

热门文章

热点

标签列表

分享到:

Copyright @ 2007~2025 All Rights Reserved.

Powered By 站长搜索

高分辨活体成像技术成功应用于活体胚胎研究

猜您喜欢

热门文章

热点

标签列表

分享到:

Copyright @ 2007~2025 All Rights Reserved.

Powered By 站长搜索